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Kirienko Lab     - we work with worms -


Contact Information

Skype: kirienkolab
Email: kirienko@rice.edu
Phone: 713-348-2581
Office: GRB Hall, W200N


         Imaging and Fluorescence Quantification in Caenorhabditis eleganswith Flow Vermimetry and Automated Microscopy.
Tjahjono E, Revtovich AV, Kirienko NV.
Bio Protoc. 2021 May 20;11(10):e4024. doi: 10.21769/BioProtoc.4024. eCollection 2021 May 20.
PMID: 34150931

Pyoverdine Inhibitors and Gallium Nitrate Synergistically Affect Pseudomonas aeruginosa.
Kang D, Revtovich AV, Deyanov AE, Kirienko NV.
mSphere. 2021 Jun 16:e0040121. doi: 10.1128/mSphere.00401-21. Online ahead of print.
PMID: 34133200

Development and Characterization of High-Throughput Caenorhabditis elegans - Enterococcus faecium Infection Model.
Revtovich AV, Tjahjono E, Singh KV, Hanson BM, Murray BE, Kirienko NV.
Front Cell Infect Microbiol. 2021 Apr 29;11:667327. doi: 10.3389/fcimb.2021.667327. eCollection 2021.
PMID: 33996637 Free PMC article.

Innate immunity in C. elegans.
Martineau CN, Kirienko NV, Pujol N.
Curr Top Dev Biol. 2021;144:309-351. doi: 10.1016/bs.ctdb.2020.12.007. Epub 2021 Mar 4.
PMID: 33992157

High-Throughput Approaches for the Identification of Pseudomonas aeruginosa Antivirulents.
Kang D, Zhang L, Kirienko NV.
mBio. 2021 May 4;12(3):e02240-20. doi: 10.1128/mBio.02240-20.
PMID: 33947765 Free article. Review.

Mitochondrial metabolism as a target for acute myeloid leukemia treatment.
Panina SB, Pei J, Kirienko NV.
Cancer Metab. 2021 Apr 21;9(1):17. doi: 10.1186/s40170-021-00253-w.
PMID: 33883040 Free PMC article. Review.

Comprehensive genetic analysis of adhesin proteins and their role in virulence of Candida albicans.
Rosiana S, Zhang L, Kim GH, Revtovich AV, Uthayakumar D, Sukumaran A, Geddes-McAlister J, Kirienko NV, Shapiro RS.
Genetics. 2021 Feb 9;217(2):iyab003. doi: 10.1093/genetics/iyab003.
PMID: 33724419 Free article.

Novel Immune Modulators Enhance Caenorhabditis elegans Resistance to Multiple Pathogens.
Hummell NA, Revtovich AV, Kirienko NV.
mSphere. 2021 Jan 6;6(1):e00950-20. doi: 10.1128/mSphere.00950-20.
PMID: 33408224 Free PMC article.

An In Vitro Cell Culture Model for Pyoverdine-Mediated Virulence.
Kang D, Kirienko NV.
Pathogens. 2020 Dec 24;10(1):9. doi: 10.3390/pathogens10010009.
PMID: 33374230 Free PMC article.

A Simple Nematode Infection Model for Studying Candida albicans Pathogenesis.
Kim GH, Rosiana S, Kirienko NV, Shapiro RS.
Curr Protoc Microbiol. 2020 Dec;59(1):e114. doi: 10.1002/cpmc.114.
PMID: 32975912

Identification and validation of a novel anti-virulent that binds to pyoverdine and inhibits its function.
Wang X, Kleerekoper Q, Revtovich AV, Kang D, Kirienko NV.
Virulence. 2020 Dec;11(1):1293-1309. doi: 10.1080/21505594.2020.1819144.
PMID: 32962519 Free PMC article.

The evolutionarily conserved ESRE stress response network is activated by ROS and mitochondrial damage.
Tjahjono E, McAnena AP, Kirienko NV.
BMC Biol. 2020 Jun 29;18(1):74. doi: 10.1186/s12915-020-00812-5.
PMID: 32600387 Free PMC article.

An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity.
Pei J, Panina SB, Kirienko NV.
J Vis Exp. 2020 May 12;(159). doi: 10.3791/61295.
PMID: 32478749

Utilizing Synergistic Potential of Mitochondria-Targeting Drugs for Leukemia Therapy.
Panina SB, Pei J, Baran N, Konopleva M, Kirienko NV.
Front Oncol. 2020 Apr 3;10:435. doi: 10.3389/fonc.2020.00435. eCollection 2020.
PMID: 32318340 Free PMC article.

Repurposing bioactive compounds for treating multidrug-resistant pathogens.
Hummell NA, Kirienko NV.
J Med Microbiol. 2020 Jun;69(6):881-894. doi: 10.1099/jmm.0.001172.
PMID: 32163353 Free PMC article.

Editorial: Beyond Antimicrobials: Non-traditional Approaches to Combating Multidrug-Resistant Bacteria.
Kirienko NV, Rahme L, Cho YH.
Front Cell Infect Microbiol. 2019 Oct 11;9:343. doi: 10.3389/fcimb.2019.00343. eCollection 2019.
PMID: 31681623 Free PMC article. No abstract available.

Pyoverdine-Dependent Virulence of Pseudomonas aeruginosa Isolates From Cystic Fibrosis Patients.
Kang D, Revtovich AV, Chen Q, Shah KN, Cannon CL, Kirienko NV.
Front Microbiol. 2019 Sep 6;10:2048. doi: 10.3389/fmicb.2019.02048. eCollection 2019.
PMID: 31551982 Free PMC article.

A mechanism for increased sensitivity of acute myeloid leukemia to mitotoxic drugs.
Panina SB, Baran N, Brasil da Costa FH, Konopleva M, Kirienko NV.
Cell Death Dis. 2019 Aug 13;10(8):617. doi: 10.1038/s41419-019-1851-3.
PMID: 31409768 Free PMC article.

Interplay between mitochondria and diet mediates pathogen and stress resistance in Caenorhabditis elegans.
Revtovich AV, Lee R, Kirienko NV.
PLoS Genet. 2019 Mar 13;15(3):e1008011. doi: 10.1371/journal.pgen.1008011. eCollection 2019 Mar.
PMID: 30865620 Free PMC article.

Novel Pyoverdine Inhibitors Mitigate Pseudomonas aeruginosa Pathogenesis.
Kirienko DR, Kang D, Kirienko NV.
Front Microbiol. 2019 Jan 9;9:3317. doi: 10.3389/fmicb.2018.03317. eCollection 2018.
PMID: 30687293 Free PMC article.

A High-throughput, High-content, Liquid-based C. elegans Pathosystem.
Anderson QL, Revtovich AV, Kirienko NV.
J Vis Exp. 2018 Jul 1;(137):58068. doi: 10.3791/58068.
PMID: 30010665 Free PMC article.

Interdependence between iron acquisition and biofilm formation in Pseudomonas aeruginosa.
Kang D, Kirienko NV.
J Microbiol. 2018 Jul;56(7):449-457. doi: 10.1007/s12275-018-8114-3. Epub 2018 Jun 14.
PMID: 29948830 Free PMC article. Review.

Pyoverdine, a siderophore from Pseudomonas aeruginosa, translocates into C. elegans, removes iron, and activates a distinct host response.
Kang D, Kirienko DR, Webster P, Fisher AL, Kirienko NV.
Virulence. 2018 Dec 31;9(1):804-817. doi: 10.1080/21505594.2018.1449508.
PMID: 29532717 Free PMC article.

PqsA Promotes Pyoverdine Production via Biofilm Formation.
Kang D, Turner KE, Kirienko NV.
Pathogens. 2017 Dec 25;7(1):3. doi: 10.3390/pathogens7010003.
PMID: 29295589 Free PMC article.

A CRISPR-Cas9-based gene drive platform for genetic interaction analysis in Candida albicans.
Shapiro RS, Chavez A, Porter CBM, Hamblin M, Kaas CS, DiCarlo JE, Zeng G, Xu X, Revtovich AV, Kirienko NV, Wang Y, Church GM, Collins JJ.
Nat Microbiol. 2018 Jan;3(1):73-82. doi: 10.1038/s41564-017-0043-0. Epub 2017 Oct 23.
PMID: 29062088 Free PMC article.

High-Throughput Genetic Screen Reveals that Early Attachment and Biofilm Formation Are Necessary for Full Pyoverdine Production by Pseudomonas aeruginosa.
Kang D, Kirienko NV.
Front Microbiol. 2017 Sep 5;8:1707. doi: 10.3389/fmicb.2017.01707. eCollection 2017.
PMID: 28928729 Free PMC article.

A conserved mitochondrial surveillance pathway is required for defense against Pseudomonas aeruginosa.
Tjahjono E, Kirienko NV.
PLoS Genet. 2017 Jun 29;13(6):e1006876. doi: 10.1371/journal.pgen.1006876. eCollection 2017 Jun.
PMID: 28662060 Free PMC article.

A High-Content, Phenotypic Screen Identifies Fluorouridine as an Inhibitor of Pyoverdine Biosynthesis and Pseudomonas aeruginosa Virulence.
Kirienko DR, Revtovich AV, Kirienko NV.
mSphere. 2016 Aug 24;1(4):e00217-16. doi: 10.1128/mSphere.00217-16. eCollection 2016 Jul-Aug.
PMID: 27579370 Free PMC article.

Mitophagy confers resistance to siderophore-mediated killing by Pseudomonas aeruginosa.
Kirienko NV, Ausubel FM, Ruvkun G.
Proc Natl Acad Sci U S A. 2015 Feb 10;112(6):1821-6. doi: 10.1073/pnas.1424954112. Epub 2015 Jan 26.
PMID: 25624506 Free PMC article.

Mitochondrial UPR-regulated innate immunity provides resistance to pathogen infection.
Pellegrino MW, Nargund AM, Kirienko NV, Gillis R, Fiorese CJ, Haynes CM.
Nature. 2014 Dec 18;516(7531):414-7. doi: 10.1038/nature13818. Epub 2014 Sep 28.
PMID: 25274306 Free PMC article.

Pseudomonas aeruginosa PA14 pathogenesis in Caenorhabditis elegans.
Kirienko NV, Cezairliyan BO, Ausubel FM, Powell JR.
Methods Mol Biol. 2014;1149:653-69. doi: 10.1007/978-1-4939-0473-0_50.
PMID: 24818940

High-throughput screening for novel anti-infectives using a C. elegans pathogenesis model.
Conery AL, Larkins-Ford J, Ausubel FM, Kirienko NV.
Curr Protoc Chem Biol. 2014 Mar 14;6(1):25-37. doi: 10.1002/9780470559277.ch130160.
PMID: 24652621 Free PMC article.

The conserved PBAF nucleosome-remodeling complex mediates the response to stress in Caenorhabditis elegans.
Kuzmanov A, Karina EI, Kirienko NV, Fay DS.
Mol Cell Biol. 2014 Mar;34(6):1121-35. doi: 10.1128/MCB.01502-13. Epub 2014 Jan 13.
PMID: 24421384 Free PMC article.

DAF-16 employs the chromatin remodeller SWI/SNF to promote stress resistance and longevity.
Riedel CG, Dowen RH, Lourenco GF, Kirienko NV, Heimbucher T, West JA, Bowman SK, Kingston RE, Dillin A, Asara JM, Ruvkun G.
Nat Cell Biol. 2013 May;15(5):491-501. doi: 10.1038/ncb2720. Epub 2013 Apr 21.
PMID: 23604319 Free PMC article.

Pseudomonas aeruginosa disrupts Caenorhabditis elegans iron homeostasis, causing a hypoxic response and death.
Kirienko NV, Kirienko DR, Larkins-Ford J, Wählby C, Ruvkun G, Ausubel FM.
Cell Host Microbe. 2013 Apr 17;13(4):406-16. doi: 10.1016/j.chom.2013.03.003.
PMID: 23601103 Free PMC article.

Stimulation of host immune defenses by a small molecule protects C. elegans from bacterial infection.
Pukkila-Worley R, Feinbaum R, Kirienko NV, Larkins-Ford J, Conery AL, Ausubel FM.
PLoS Genet. 2012;8(6):e1002733. doi: 10.1371/journal.pgen.1002733. Epub 2012 Jun 14.
PMID: 22719261 Free PMC article.

Host translational inhibition by Pseudomonas aeruginosa Exotoxin A Triggers an immune response in Caenorhabditis elegans.
McEwan DL, Kirienko NV, Ausubel FM.
Cell Host Microbe. 2012 Apr 19;11(4):364-74. doi: 10.1016/j.chom.2012.02.007.
PMID: 22520464 Free PMC article.

Genomic and Experimental Evidence Suggests that Verrucomicrobium spinosum Interacts with Eukaryotes.
Sait M, Kamneva OK, Fay DS, Kirienko NV, Polek J, Shirasu-Hiza MM, Ward NL.
Front Microbiol. 2011 Oct 18;2:211. doi: 10.3389/fmicb.2011.00211. eCollection 2011.
PMID: 22022322 Free PMC article.

Cancer models in Caenorhabditis elegans.
Kirienko NV, Mani K, Fay DS.
Dev Dyn. 2010 May;239(5):1413-48. doi: 10.1002/dvdy.22247.
PMID: 20175192 Free PMC article. Review.

SLR-2 and JMJC-1 regulate an evolutionarily conserved stress-response network.
Kirienko NV, Fay DS.
EMBO J. 2010 Feb 17;29(4):727-39. doi: 10.1038/emboj.2009.387. Epub 2010 Jan 7.
PMID: 20057358 Free PMC article.

Structure and mechanism of a bacterial light-regulated cyclic nucleotide phosphodiesterase.
Barends TR, Hartmann E, Griese JJ, Beitlich T, Kirienko NV, Ryjenkov DA, Reinstein J, Shoeman RL, Gomelsky M, Schlichting I.
Nature. 2009 Jun 18;459(7249):1015-8. doi: 10.1038/nature07966.
PMID: 19536266

Coordinated regulation of intestinal functions in C. elegans by LIN-35/Rb and SLR-2.
Kirienko NV, McEnerney JD, Fay DS.
PLoS Genet. 2008 Apr 25;4(4):e1000059. doi: 10.1371/journal.pgen.1000059.
PMID: 18437219 Free PMC article.

Transcriptome profiling of the C. elegans Rb ortholog reveals diverse developmental roles.
Kirienko NV, Fay DS.
Dev Biol. 2007 May 15;305(2):674-84. doi: 10.1016/j.ydbio.2007.02.021. Epub 2007 Feb 21.
PMID: 17368442 Free PMC article.

lin-35/Rb and the CoREST ortholog spr-1 coordinately regulate vulval morphogenesis and gonad development in C. elegans.
Bender AM, Kirienko NV, Olson SK, Esko JD, Fay DS.
Dev Biol. 2007 Feb 15;302(2):448-62. doi: 10.1016/j.ydbio.2006.09.051. Epub 2006 Oct 5.
PMID: 17070797 Free PMC article.

Significance of codon usage and irregularities of rare codon distribution in genes for expression of BspLU11III methyltransferases.
Kirienko NV, Lepikhov KA, Zheleznaya LA, Matvienko NI.
Biochemistry (Mosc). 2004 May;69(5):527-35. doi: 10.1023/b:biry.0000029851.96180.92.
PMID: 15193127