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Research Interests:
Our lab has previously found that the bacteria
filtrate has toxicity towards murine
macrophages. This cytotoxicity could be
associated with the outer membrane vesicles (OMVs)
produced by Pseudomonas
aeruginosa.
I am interested in the role that OMVs play in
cytotoxicity as well as infection. Currently I
am trying to establish a purification system
with high reproducibility. The cytotoxicity of
purified vesicles will then be verified in a
variety of cells. I will further investigate the
characteristics of the vesicles in multiple
methods, including particle measurement, protein
analysis, fluorescence microscopy and lipid
analysis. Afterward, I will expand the strain
choice, in order to better understand the
functions of produced vesicles. This might
reveal the mechanism for the severity and
widespread of the infections caused by P.
aeruginosa in
clinic.
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